Ōsasahara Shrine (大笹原神社, Ōsasahara Jinja) is a Shinto shrine located in the city of Yasu, Shiga Prefecture, Japan.

The kami worshipped at this shrine are Susanoo-no-Mikoto and Kushinadahime.

Ōsasahara Shrine was constructed in the year 986 by Echi Morozane, a local warlord.

In 1414, the shrine's main hall (honden) was reconstructed by Mabuchi Sadanobu, the castellan of Iwakura Castle.

Following the Meiji restoration and the establishment of the Modern system of ranked Shinto shrines under State Shinto, the shrine was officially designated a "county shrine".