The senior side was a long-term member of the Oberliga Südwest from 1945, then a tier one league, to 1963 when the Bundesliga was introduced.

The reserve side, in the early years after the Second World War, played mostly in local amateur football.

[1] The club spent the next four seasons in the Oberliga Südwest as a top of the table side, culminating in a second-place finish in 2002 and a league championship the year after.

[3] In this era the club also saw five consecutive South West Cup wins from 2001 to 2005, a competition the team has been barred from like all reserve sides in Germany, following a rule change in 2008.

Another three good seasons in the Oberliga Südwest followed in which Mainz 05 II finished third, second and, eventually in 2008, league champions again.

[2] This time promotion took the club up to the Regionalliga West, a newly introduced league that was formed alongside the introduction of the 3.

Because runners-up SC Freiburg II declined the opportunity to take part in the promotion round, choosing not to apply for a 3.

Liga after overcoming Regionalliga Nordost champions TSG Neustrelitz 5–1 on aggregate in the promotion round.

[7] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.