The 125 FW is also available to other combatant commanders for forward deployment outside the United States (OCONUS) in order to perform air superiority/air dominance missions in other theaters of operation and areas of responsibility.

In addition to its primary installation, the Jacksonville Air National Guard Base at Jacksonville International Airport, the 125 FW also maintains an alert detachment of F-15 Eagle fighter aircraft at Detachment 1, 125 FW, Operating Location ALPHA ALPHA (OL-AA) at Homestead Air Reserve Base in South Florida.

The overall wing organization comprises over 1100 citizen airmen in a combination of full-time Active Guard and Reserve (AGR) and Air Reserve Technician (ART) personnel and traditional part-time Drill Status Air Guardsmen (DSG), also known as Traditional Guardsmen (TG).

This same alert detachment continues operations today at the present day Homestead Air Reserve Base with no less than two 125th F-15C aircraft, pilots, and support personnel periodically rotated from home station in Jacksonville.

On a daily basis in the United States, the 125 FW is responsible for the maintenance of a USNORTHCOM / NORAD Air Defense Alert mission at both its home station in Jacksonville ANGB and at an additional operating location at Homestead ARB, Florida.

In the past, this threat has included Soviet Tu-95 BEAR and Tu-22 BLACKJACK bombers, various Soviet-built Cuban Air Force fighters, and civilian narcotics traffickers of various nationalities.

The 125 FW was rated "Outstanding" during an Alert Force Evaluation by the North American Aerospace Defense Command Inspector General in November 1997.

As the 125 FIG, 125 FG and 125 FW, the unit subsequently operated a single C-130E Hercules, followed by a single WC-130H Hercules aircraft (the latter with weather reconnaissance equipment removed) for logistical support of the wing, geographically separated units (GSUs) of the Florida Air National Guard, and additional "as needed" airlift support to the Florida Army National Guard.

This capability is currently gapped in the Florida Air National Guard until another similar support aircraft can be assigned with fixed-wing aviation elements of the Florida Army National Guard currently providing principal OSA assets from an aviation support facility in St. Augustine.

The 125 FW also operates a single RC-26B Metroliner reconnaissance aircraft in support of state and federal law enforcement agencies under Title 32 USC authority.

The 101st Air and Space Operations Group and HQ FLANG Detachment 1 at Tyndall AFB were unaffected by these changes.

On 27 July 2021, the Department of the Air Force signed a Record of Decision announcing that the 125 FW will eventually receive twenty (20) F–35A Lightning II aircraft to replace its extant F-15Cs and F-15Ds.

The 125 FW continues to regularly participates in RED FLAG events at the USAF Warfare Center at Nellis AFB, Nevada and the Exercise SENTRY SAVANNAH[7] and the WILLIAM TELL air-to-air weapons meet at the Georgia Air National Guard's Air Dominance Center at Savannah ANGB as part of its on-going readiness program.