The regiment was organized at Camp Wood, Quincy, Illinois and mustered in for one-hundred day service on June 21, 1864,[2] with Colonel J.W.

[3] The Secretary of War, Edwin M. Stanton, ordered the regiment to proceed to Fort Leavenworth, Kansas, where it was assigned to garrison duty.

Following the defeat of the bushwhackers, Companies "C" and "F" returned to garrison duty at Fort Leavenworth.

[3] The regiment voluntarily extended its term of service and proceeded along the Iron Mountain Railroad guarding railway bridges from destruction by Price's Army.

[3] Following the defeat of General Price's army, the regiment returned to Camp Butler, Illinois and was mustered out on October 14, 1864.