It was subsequently re-mustered for three months federal service under the command of Colonel Isaac Ferdinand Quinby.

The regiment was transferred from state service to United States service for the balance of their term by order of Governor Edwin D. Morgan in August 2, 1861.

The regiment was attached to Sherman's Brigade, Tyler's Division, McDowell's Army of Northeast Virginia, from June to August 1861.

For Martindale's Brigade, Porter's Division, Army of the Potomac, to March 1862.

For 1st Brigade, 1st Division, III Corps, Army of the Potomac, to May 1862.

Men who had enlisted for three years' service were consolidated into two companies and served duty as Provost Guard, 1st Division, V Corps, April 27 to June 23.

These two companies ceased to exist on June 23, 1863 when its members were transferred to the 140th New York Infantry.

Camp on Meridian Hill, defenses of Washington, until June 3, 1861, and at Fort Corcoran until July 16.

White Oak Swamp and Turkey Bend June 30.