The 141st New York Infantry was organized at Elmira, New York, beginning August 14, 1862 and mustered in for three years service on September 11, 1862 under the command of Colonel Samuel G. Hathaway Jr..

The regiment was attached to VIII Corps, Middle Department, to October 1862.

2nd Brigade, Abercrombie's Division, Defenses of Washington, to February 1863.

2nd Brigade, Abercrombie's Division, XXII Corps, to April 1863.

1st Brigade, 3rd Division, VII Corps, Department of Virginia, to May 1863.

1st Brigade, 1st Division, XX Corps, Army of the Cumberland, to June 1865.

Recruits and veterans were transferred to the 60th New York Infantry.

Left New York for Middle Department September 15, 1862.

March in pursuit of Lee to Berlin, Md., July 13–24.

Duty along Orange & Alexandria Railroad until September.

Battles about Dallas, New Hope Church, and Allatoona Hills May 26-June 5.

Operations about Marietta and against Kennesaw Mountain June 10-July 2.

Ruff's Station, Smyrna Camp Ground, July 4.

Operations at Chattahoochie River Bridge August 26-September 2.