The 14th Ohio Infantry Regiment was an infantry regiment in the Union Army during the American Civil War.

The 14th Ohio Infantry Regiment was organized at Toledo, Ohio, on April 25, 1861, under Colonel James Blair Steedman in response to President Lincoln's call for 75,000 volunteers.

The 14th Ohio Infantry was reorganized at Toledo on August 14-September 5, 1861, and mustered in for three years service.

[1] The 14th Ohio Infantry mustered out of service at Louisville, Kentucky, on July 11, 1865.

The regiment lost a total of 332 men during service; 5 officers and 141 enlisted men killed or mortally wounded, 1 officer and 185 enlisted men died of disease.