The squadron initially operated fifteen MD500s and three AH-1S Cobras.

The squadron conducted operations during Operation Peace for Galilee, Israel's 1982 invasion of Lebanon, but did not initially engage in anti-tank combat, rather serving as close air support.

In 2001, Israel acquired 15 AH-1E helicopters from the US Army.

The squadron was based at Palmachim Airbase.

On 2 August 2013, as part of the IDF's budget cut plans, along with the fact that the Cobra helicopters were considered relatively unsafe with many accidents credited to its operation history, it was closed (along with 140 Squadron "Golden Eagle",) In 2020, the 160 Squadron was reopened at Ramat David Airbase as Shadow Hunter Squadron, operating classified UAVs.