The 160th New York Infantry was organized at Auburn, New York beginning September 6, 1862 and mustered in for three-years service on November 21, 1862 under the command of Colonel Charles C. Dwight.

The regiment was attached to Sherman's Division, Department of the Gulf, to January 1863.

2nd Brigade, 1st Division, XIX Corps, Department of the Gulf, to July 1863.

3rd Brigade, 1st Division, XIX Corps, Army of the Gulf, to July 1864, and Army of the Shenandoah, Middle Military Division, to February 1865.

3rd Brigade, 1st Provisional Division, Army of the Shenandoah, to April 1865.

3rd Brigade, Dwight's Division, Department of Washington, to June 1865.

3rd Brigade, Dwight's Division, Department of the South, to November 1865.

The 160th New York Infantry mustered out of service November 1, 1865.

Kock's Plantation, Donaldsonville, on Bayou Fourche, July 13–14.

Monett's Ferry or Cane River Crossing April 23.

Served detached as supply train guard for the army from August 14 to October 27.

Duty at Middletown and Newtown until December, and at Stephenson's Depot and Winchester until April 1865.