This unit has a long and distinguished history that lives up to the Medical Branch's tradition of being faithful in adversity.

It is the latest in a line of Saskatchewan army medical units dating back to 21 Field Ambulance of World War I and 10 Field Ambulance of World War II.

From 1940 to 1941, the unit trained at Camp Dundurn, in preparation for deployment to England.

In the early 1970s, the unit was disbanded along with all militia medical units across Canada, but was reformed as a Medical Company, as part of 16 Service Battalion, a combat service support unit, based out of Regina Garrison in Regina, Saskatchewan.

16 (Regina) Field Ambulance is affiliated with 328 Royal Canadian Army Medical Cadet Corps,[1] based in Saskatoon.