The 16th Annual Helpmann Awards for live performance in Australia were held on 25 July 2016 at the Sydney Lyric Theatre.

Griffin Theatre Company's drama The Bleeding Tree received three awards including Best Play.

Brisbane Baroque's production of Handel's Agrippina received four awards including Best Opera, and Bangarra Dance Theatre's Sheoak received three awards including Best Ballet or Dance Work.

New musical Ladies in Black was named Best New Australian Work.

In the following tables, winners are listed first and highlighted in boldface.