The 16th Wisconsin Infantry Regiment was a volunteer infantry regiment that served in the Union Army during the American Civil War.

For much of the war, the regiment was commanded by Cassius Fairchild, the brother of Wisconsin's 10th governor Lucius Fairchild.

The 16th Wisconsin suffered 6 officers and 141 enlisted men killed in action or who later died of their wounds, plus another 4 officer and 248 enlisted men who died of disease, for a total of 399 fatalities.

[1] The six-man color guard were all killed on April 6, 1862.

They are memorialized with cenotaphs at what was the apex of the Shiloh Military Cemetery overlooking the Tennessee River.