The regiment spent its entire enlistment engaged in guard government stores at Gallipolis, Ohio.
Over 35,000 Ohio National Guardsmen were federalized and organized into regiments for 100 days service in May 1864.
Shipped to the Eastern Theater, they were designed to be placed in "safe" rear areas to protect railroads and supply points, thereby freeing regular troops for Lt. Gen. Ulysses S. Grant’s push on the Confederate capital of Richmond, Virginia.
Ohio Guard units met the battle-tested foe head on and helped blunt the Confederate offensive thereby saving Washington, D.C. from capture.
Ohio National Guard units participated in the battles of Monacacy, Fort Stevens, Harpers Ferry, and in the siege of Petersburg.