Despite the final implications of jumping the hurdle from amateur to professional status during the previous season, Brentford went into the 1900–01 Southern League Second Division campaign in a healthy state.
[1] Now under the charge of William Lewis as a team manager of sorts, Brentford trimmed down the playing staff and signed two new professional players – left back Harry Gilmour and inside forward Joe Turner.
[1] McLeod and Turnbull proved to be master-stroke signings and along with existing forwards Ralph McElhaney, E. Andrews and Joe Turner, the quintet fired Brentford to an unbeaten Southern League season.
[2] With Brentford needing to win to secure promotion and with the score at 0–0, the match at Elm Park went into extra time and was called off after 107 minutes due to bad light.
[3] In the aftermath of the abandoned test match, the Brentford committee resolved to apply for the place in the First Division left vacant by Bristol City, who had been elected into the Football League.