The Netherlands Football League Championship 1900–1901 was contested by fifteen teams participating in two divisions.
The national champion would be determined by a play-off match featuring the winners of the eastern and western football division of the Netherlands.
HVV Den Haag won this year's championship by beating Victoria Wageningen 2–1 in a decision match.
Eerste Klasse East: Eerste Klasse West: HVV Den Haag won the championship.