The 1900–01 Northern Rugby Football Union season was the sixth season of rugby league football.

In 1901 Bramley's James Lomas became rugby league's first £100 transfer, moving to Salford.

Although participating in the Lancashire Senior Competition, Runcorn and Stockport were from Cheshire, and Millom were from Cumberland.

The 1901 Challenge Cup was the 5th staging of rugby league's oldest knockout competition, the Challenge Cup.

[2] The final was played between Batley and Warrington at Headingley Stadium in Leeds.