The league consisted of Eastern and Western Divisions.
[1] Kettering Town won the Eastern Division and Bristol City reserves won the Western Division.
Kettering were declared Southern League champions after defeating Bristol City reserves 5–0 in a championship play-off.
Three clubs (all from the Western Division) left the league at the end of the season.
Team relegated from 1926–27 Football League Newly elected teams: Two Southern League clubs, Kettering Town and Peterborough & Fletton United, applied to join the Football League.