After suffering a 0–12 upset loss at the hands of the Davidson College Wildcats, the Gators rallied to defeat the Auburn Tigers 33–6, defeating the Tigers for the first time and ending a six-game losing streak, and to upset coach Wallace Wade's Alabama Crimson Tide 13–6.

[3][4] With Middlekauff at captain, the Gators suffered just two further losses: to conference co-champions NC State; and to Georgia's "Dream and Wonder team".

[18] On October 4, captain Frank Oosterhoudt was declared ineligible; his replacement by unanimous vote was Bill Middlekauff.

Florida's final touchdown was on a triple-pass play of Middlekauff to Clyde Crabtree to Dale Van Sickel.

[28] Goof Bowyer scored a touchdown and drop kicked two extra points to seal the first victory over the Wildcats.

[30] In the fifth week of play, the Gators faced coach Gus Tebell's North Carolina State Wolfpack on Plant Field in Tampa, losing 12–6.

[31] A drive brought the Wolfpack to the 3-yard line, the feature play of which was a 30-yard pass from their Hall of Fame captain, Gainesville native Jack McDowall to Childress.

A pass bounced off the hands of a Florida back and into McDowall's, who returned the ball 75 yards for the deciding score.

[32][34][35] Since McDowall had been turned down by his native University of Florida, legend has it just afterwards he mailed Sebring the game ball.

Florida's five touchdowns were scored by: Bill Middlekauff, Willie DeHoff, Cecil Beck, Spic Stanley, and Goof Bowyer.

This pass started out like a cross buck but finished with Johnson having tossed the ball to McCrary over the goal line.

[46] Before a crowd of some 12,000 in Jacksonville, Florida beat coach Pat Herron's Washington & Lee Generals 20–7.

[48] In a cold and driving rain, a placekick for extra point by Dutch Stanley proved the difference in a 7–6 win over coach Curley Byrd's Maryland Terrapins.

[49][50] "Playing on a sodden field, unfamiliar to both teams,"[51] Florida scored after Clyde Crabtree returned a punt to Maryland's 38-yard line.

The following chart provides a visual depiction of Florida's lineup during the 1927 season with games started at the position reflected in parentheses.