First-seeded Helen Jacobs defeated Carolin Babcock 6–2, 6–2 in the final to win the women's singles tennis title at the 1932 U.S. National Championships.

[1][2] The tournament used two lists of players for seeding the women's singles event; one list of eight U.S. players and a list for three foreign players.

Helen Jacobs is the champion; others show in brackets the round in which they were eliminated.