First-seeded Helen Jacobs defeated second-seeded Sarah Palfrey 6–1, 6–4 in the final to win the women's singles tennis title at the 1934 U.S. National Championships.

[1][2] The tournament used two lists of four players for seeding the women's singles event; one for U.S. players and one for foreign players.

Helen Jacobs is the champion; others show in brackets the round in which they were eliminated.