The competition is notable for the exploits of Manchester United following the loss of much of their team in the Munich air disaster on 6 February 1958.
If scores were level after 90 minutes had been played, a replay would take place at the stadium of the second-named team later the same week.
Those appearing in the competition proper for the first time were Prescot Cables, Spalding United, Redhill and Walton & Hersham.
To complete this round, Bishop Auckland and Wycombe Wanderers were given byes as the champions and runners-up from the previous season's FA Amateur Cup.
Leeds United and Cardiff City were meeting in this round at Elland Road for the third consecutive year, with the Welsh club winning each of the matches by the same 2-1 scoreline.
The FA Cup final took place on 3 May 1958 at Wembley Stadium and was won by Bolton Wanderers, beating Manchester United 2–0.
United had been devastated by the Munich air disaster, but still won through three rounds of the cup following the incident, before meeting Bolton in the final.