The 1960 Cal Poly Mustangs football team represented California Polytechnic State College as a member of the California Collegiate Athletic Association (CCAA) during the 1960 college football season.
Led by 11th-year head coach LeRoy Hughes, Cal Poly compiled an overall record of 1–5 with a mark of 1–4 in conference play, placing fifth in the CCAA.
Tragedy struck following the game at Bowling Green State University on October 29, when the aircraft transporting the team crashed, killing 22 people, including 16 Cal Poly Mustang football players and the team manager.
Los Angeles State and UC Santa Barbara were awarded forfeits from the Mustangs in the CCAA standings, but these results were not reflected in the overall records for the teams.
The following year, on November 23, 1961, a benefit game was held to raise a memorial fund for the survivors and bereaved families.