Matches were scheduled to be played at the stadium of the team named first on the date specified for each round, which was always a Saturday.
If scores were level after 90 minutes had been played, a replay would take place at the stadium of the second-named team later the same week.
The final two non-league sides in the main draw, Wimbledon and Sutton United were given byes to this round as the champions and runners-up from the previous season's FA Amateur Cup.
Oxford United was appearing at this stage for the first time in only their second season as a Football League club.
Preston North End and West Ham United came through the semi-final round to meet at Wembley.
The 1964 FA Cup final was contested by Preston North End and West Ham United at Wembley on Saturday, 2 May 1964.