The Dutch Tweede Divisie in the 1963–64 season was contested by 32 teams, divided in two groups.
NEC won the championship after beating Alkmaar '54 in a play-off.
Roda JC qualified for the Best 3rd Place play-off owing to a superior Goal Average in the "regular season".
Roda JC entered the promotion tournament.
Entering teams: However, as KFC & Be Quick 1887 both relegated themselves, LONGA retained their spot.