The 1964–65 Taça de Portugal was the 25th edition of the Portuguese football knockout tournament, organized by the Portuguese Football Federation (FPF).
[3][4] Teams from the Primeira Liga (I) and the Portuguese Second Division (II) entered at this stage.
In case the aggregate score after the two games was level, the cup tie would be replayed.
[5][6] Due to the odd number of teams involved at this stage of the competition, Oriental qualified for the next round due to having no opponent to face at this stage of the competition.
[7][8] Due to the odd number of teams involved at this stage of the competition, Belenenses qualified for the next round due to having no opponent to face at this stage of the competition.