The Dutch Tweede Divisie in the 1964–65 season was contested by 31 teams, sixteen of which playing in group A, fifteen in group B. DFC won the championship after beating Cambuur Leeuwarden in a playoff.
This way, it turned out that Xerxes (the number three of group B) would be promoted to the Eerste Divisie after SC Cambuur.
Acquired football team: Entered from the B-group: (Leeuwarden would play as SC Cambuur from this season onwards) Relegated from the Eerste Divisie: Entered from the A-group:
Entering teams: However, LONGA voluntarily relegated themselves to amateur football after the game.
Therefore, a replay, as well as a relegation play-off (involving bottom-finishers Zwolsche Boys from Group A), did not have to be played (while HVV 't Gooi moved to Tweede Group A next season).