1964 European Nations' Cup round of 16

It was contested by the thirteen winners from the preliminary round, along with Austria, Luxembourg and the Soviet Union, who had received a bye.

The winners of each of eight home-and-away ties progressed to the quarter-finals.

The round of 16 was played in two legs on a home-and-away basis.

Republic of Ireland won 3–2 on aggregate and advanced to the quarter-finals.

Soviet Union won 3–1 on aggregate and advanced to the quarter-finals.