Everton won the competition for the third time, beating Sheffield Wednesday 3–2 in the final at Wembley.
If scores were level after 90 minutes had been played, a replay would take place at the stadium of the second-named team later the same week.
Corinthian-Casuals was the only qualifying club appearing in the main competition for the first time, although the two predecessor outfits Corinthian and Casuals had both featured at this stage prior to their merger in 1939.
Of the others, Burton Albion had last progressed through to the first round in 1956-57, Leytonstone in 1952-53, Fleetwood and Wealdstone in 1949-50, and Wellingborough Town in 1928-29.
The final two non-league sides in the main draw, Hendon and Barnet received byes to this round, Hendon as the champions from the previous season's FA Amateur Cup and Barnet as the best-performed non-league club in the previous FA Cup tournament.
The 1966 FA Cup final was contested by Sheffield Wednesday and Everton at Wembley on Saturday 14 May 1966.
Everton were looking to become the first team since Bury won in 1903 to win the cup without conceding a goal, while Sheffield Wednesday were the second Yorkshire side in succession to reach Wembley, following Leeds United's losing appearance in the 1965 final.