1965–66 Regionalliga

The league operated in five regional divisions, Berlin, North, South, Southwest and West.

The five league champions and four runners-up, the Regionalliga Berlin runners-up was not qualified, entered a promotion play-off to determine the two clubs to move up to the Bundesliga for the next season.

The two promotion spots went to the Regionalliga West champions Fortuna Düsseldorf and runners-up Rot-Weiß Essen.

FC Neukölln, VfB Hermsdorf, Lichterfelder SU, SC Tegel, SC Gatow and SC Staaken, all promoted from the Amateurliga as the Regionalliga had been expanded from 10 to 16 clubs, while Hertha BSC Berlin had been relegated from the Bundesliga to the league.

The 1965–66 season saw three new clubs in the league, Opel Rüsselsheim, VfR Pforzheim and SpVgg Weiden, all promoted from the Amateurliga, while no club had been relegated from the Bundesliga to the league.