1965–66 Taça de Portugal

Unlike previous editions, two legged first round cup ties were abolished which meant that each cup tie would be contested over one leg.

Teams from the Primeira Liga (I) and the Portuguese Second Division (II) entered at this stage.

Due to the odd number of teams involved at this stage of the competition, Cova da Piedade qualified for the next round due to having no opponent to face at this stage of the competition.

Due to the odd number of teams involved at this stage of the competition, Beira-Mar and Vitória de Setúbal qualified for the next round due to having no opponent to face at this stage of the competition.

Lusitânia, Marítimo, and Mindelense were invited to participate in the competition.