The 1965 Bucknell Bison football team was an American football team that represented Bucknell University as a member of the University Division of the Middle Atlantic Conference (MAC) during the 1965 NCAA College Division football season.
In their first year under head coach Carroll Huntress, the Bison compiled an overall record of 6–3 with a mark of 5–1 in conference play, winning the MAC University Division title.
18 in the UPI small college poll, but lost that week to Penn and dropped out of the rankings.
Despite recovering with a conference-winning record, they remained unranked through the end of the year.
Bucknell played home games at Memorial Stadium on the university's campus in Lewisburg, Pennsylvania.