The 1965 Five Nations Championship was the thirty-sixth series of the rugby union Five Nations Championship.

Including the previous incarnations as the Home Nations and Five Nations, this was the seventy-first series of the northern hemisphere rugby union championship.

Ten matches were played between 9 January and 27 March.

It was contested by England, France, Ireland, Scotland and Wales.

Wales missed out on a fourth Grand Slam after losing to France at Stade Colombes despite winning the title.