The 1965 NCAA University Division swimming and diving championships were contested at the 29th annual swim meet sanctioned and hosted by the NCAA to determine the individual and team national champions of men's collegiate swimming and diving among its University Division member programs in the United States, culminating the 1964–65 NCAA University Division swimming and diving season.

These championships were hosted by Iowa State University at the Beyer Hall Pool in Ames, Iowa during March 1964.

Two-time defending champions USC again topped the team standings, edging out Indiana by 6.5 points.

This was the Trojans' fourth national title and fourth in five seasons.

[1]