The 1965 Salisbury by-election was a by-election held for the British House of Commons constituency of Salisbury in Wiltshire on 4 February 1965.
It was won by the Conservative Party candidate Michael Hamilton.
The seat had become vacant when the 58-year-old sitting Conservative Member of Parliament (MP) John Morrison had been ennobled as Baron Margadale.
The Labour Party selected the National Union of Bank Employees official Leif Mills, and the Liberal Party fielded Hugh Capstick; both had contested the seat at the general election in October 1964.
Michael Hamilton of the Conservative Party returned to the House of Commons as an MP after he lost his Wellingborough seat in the General election in 1964.