The 20th Vuelta a España (Tour of Spain), a long-distance bicycle stage race and one of the three grand tours, was held from 29 April to 16 May 1965.

It consisted of 18 stages covering a total of 3,410 km (2,120 mi), and was won by Rolf Wolfshohl of the Mercier cycling team.

[1] Julio Jiménez won the mountains classification while Rik Van Looy won the points classification.

General classification(la roja) Points classification(jersey verde) Mountains classification(jersey puntos azules) Young rider classification(jersey blanco) Team classification(clasificación por equipos) Combativity award