64 teams contested, beginning on 2 September 1989 and ending at the final on 25 April 1990.
PSV beat Vitesse Arnhem 1–0 and won the cup for the sixth time.
E Eredivisie; 1 Eerste Divisie; A Amateur teams There was only room for 32 teams in the next round, so this intermediary round was held on October 4, 1989.
The ten highest ranked Eredivisie teams from last season entered the tournament this round.
E ten Eredivisie entrants The matches of the round of 16 were played on January 13 and 14, 1990.