Defending champion Steffi Graf defeated Martina Navratilova in the final, 3–6, 7–5, 6–1 to win the women's singles tennis title at the 1989 US Open.

This marked the final professional appearance for former world No.

She reached the quarterfinals in all 19 US Opens she entered, 17 times reaching the semifinals or better, and winning the title six times.

Future two-time champion Monica Seles made her US Open debut, losing to Evert in the fourth round.

Steffi Graf is the champion; others show the round in which they were eliminated.