The 1990–91 FA Trophy was the twenty-second season of the FA Trophy.
The teams that given byes to this round are Barrow, Colchester United, Barnet, Runcorn, Macclesfield Town, Kettering Town, Welling United, Yeovil Town, Sutton United, Merthyr Tydfil, Wycombe Wanderers, Cheltenham Town, Telford United, Kidderminster Harriers, Northwich Victoria, Altrincham, Stafford Rangers, Slough Town, Bath City, Gateshead, Farnborough Town, Aylesbury United, Dartford, Wokingham Town, Redbridge Forest, Billingham Synthonia, Woking, Dover Athletic, Hyde United, Leek Town and Gretna.