The Serie B 1993–94 was the sixty-second tournament of this competition played in Italy since its creation.

Ravenna, Vicenza, Palermo and Acireale had been promoted from Serie C, while Brescia, Fiorentina, Ancona and Pescara had been relegated from Serie A. Padova promoted to Serie A.

Played in Salerno on June 14 Pisa relegated and later went bankrupt.