The 1994–95 season was the 80th season of the Isthmian League, which is an English football competition featuring semi-professional and amateur clubs from London, East and South East England.
Division One consisted of 22 clubs, including 16 clubs from the previous season and six new clubs: Clubs relegated from the Premier Division: Clubs promoted from Division Two: At the end of the season Leyton who merged with Walthamstow Pennant and formed new club Leyton Pennant.
Division Two consisted of 22 clubs, including 16 clubs from the previous season and six new clubs: Clubs relegated from Division One: Clubs promoted from the Third Division: At the end of the season Malden Vale resigned from the league and merged into Raynes Park to form a new club Raynes Park Vale, who joined Combined Counties League.
Division Three consisted of 21 clubs, including 17 clubs from the previous season and four new clubs: At the end of the season Feltham & Hounslow resigned from the league and joined the Combined Counties League.
Also, club was renamed back into Feltham.