Steffi Graf defeated Natasha Zvereva in the final, 4–6, 6–1, 6–2 to win the women's singles tennis title at the 1994 Miami Open.

Arantxa Sánchez Vicario was the two-time defending champion, but lost in the quarterfinals to Brenda Schultz.

A champion seed is indicated in bold text while text in italics indicates the round in which that seed was eliminated.

All thirty-two seeds received a bye to the second round.