The 1995–96 season was the 81st season of the Isthmian League, which is an English football competition featuring semi-professional and amateur clubs from London, East and South East England.
The league consisted of four divisions.
At the end of the season Saffron Walden Town resigned from the league due to ground grading problems and joined the Essex Senior League.
Thus, no teams were relegated from the Second Division this season.
Division Three consisted of 21 clubs, including 17 clubs from the previous season and four new teams: Two clubs relegated from Division Two: Plus: