The 1995 NCAA Division I men's basketball tournament involved 64 schools playing in single-elimination play to determine the national champion of men's NCAA Division I college basketball.

It began on March 16, 1995, and ended with the championship game on April 3 at the Kingdome in Seattle, Washington.

The following are the sites that were selected to host each round of the 1995 tournament: First and Second Rounds Regional semifinals and finals (Sweet Sixteen and Elite Eight) National semifinals and championship (Final Four and championship) There were 29 automatic bids awarded to the tournament - of these, 26 were given to the winners of their conference's tournament, while three were awarded to the team with the best regular-season record in their conference (Big Ten, Ivy League and Pac-10).

Five conference champions made their first NCAA tournament appearances: Colgate (Patriot), FIU (TAAC), Gonzaga (West Coast), Mount St. Mary's (NEC), and Nicholls State (Southland).

# Minnesota vacated its NCAA Tournament appearance from the 1994–95 season due to an academic fraud scandal.