The 1995 Wisconsin–La Crosse Eagles football team represented the University of Wisconsin–La Crosse as a member of the Wisconsin State University Conference (WSUC) during the 1995 NCAA Division III football season.
In their 27th season under head coach Roger Harring, the Eagles compiled a 14–0 record (7–0 against conference opponents) and won the WSUC championship.
They participated in the NCAA Division III playoffs, defeating Concordia–Moorhead in the regionals, Wisconsin–River Falls in the quarterfinals, Mount Union in the semifinals, and Rowan in the Stagg Bowl to win the Division III national championship.
The team played its home games at Veterans Memorial Stadium in La Crosse, Wisconsin.