[4] The 30 winners of the previous round were joined by an additional 14 clubs from the National Conference Premier Division.

This was due to Oldham being originally excluded from the cup, as the original club had been liquidated at the end of the previous season, but the Rugby Football League (RFL) later allowed the club to enter the competition following an appeal, with several of the remaining amateur clubs in the competition offering to play Oldham for a place in the next round.

[4] The 20 winners of the previous round were joined by an additional 12 clubs from the Super League.

Sheffield Eagles won 22–18 against Salford Reds, reaching the Challenge Cup final for the first time in their history, but the match was overshadowed when a man invaded the pitch and attacked referee Stuart Cummings immediately after the end of the game.

[7] Wigan Warriors won 38–8 in a one-sided game against London Broncos.