10 teams competed in the league, 9 of which returned from the previous season and 1 of which was promoted from Primera A. Tauro were crowned champions after defeating Plaza Amador 2-0 in the Rommel Fernández becoming Tauro's fifth title in its history.

After clinching this title Tauro earned the Panama 1 spot in the 2001 season of the Copa Interclubes UNCAF while Plaza Amador earned the Panama 2 spot as the runner-up.

Previous to the start of the season there was a championship that went on from April to July amongst the participating teams to determine who would play in the 2000 season of the Copa Interclubes UNCAF; the 1999–2000 Campeonato Apertura.

Panamá Viejo was crowned champion on July 2 after defeating Tauro 2-0.

El Super Clasico Nacional - Tauro v Plaza Amador