They played their home games at Hilton Coliseum in Ames, Iowa and competed in the Big 12 Conference.

They defeated Central Connecticut, Auburn, and UCLA to reach the Elite Eight where they lost to Michigan State.

[6] The preseason Big 12 Coaches' Poll picked Iowa State to finish sixth in the conference.

On November 23, 1999, Iowa State didn't receive any votes in the USA Today/ESPN Top 25 coaches poll.

On December 14, 1999, Iowa State didn't receive any votes in the USA Today/ESPN Top 25 coaches poll.

[15] On December 21, 1999, Iowa State didn't receive any votes in the USA Today/ESPN Top 25 coaches poll.

[16] On December 28, 1999, Iowa State didn't receive any votes in the USA Today/ESPN Top 25 coaches poll.

[17] On January 4, 2000, Iowa State didn't receive any votes in the USA Today/ESPN Top 25 coaches poll.

[18] On January 11, 2000, Iowa State received 1 point in the USA Today/ESPN Top 25 coaches poll which had them ranked T–47th.

[20] On January 18, 2000, Iowa State received 6 points in the USA Today/ESPN Top 25 coaches poll which had them ranked T–39th.

On March 13, 2000, Iowa State announced that head coach Larry Eustachy had signed a 10-year contract extension.

[40] On March 30, 2000, Jamaal Tinsley announced he had elected to stay at Iowa State for his senior season.

[42] On April 4, 2000, Iowa State received 657 points in the final USA Today/ESPN Top 25 coaches poll which had them ranked 3rd.