The 1999–2000 Scottish Inter-District Championship was a rugby union competition for Scotland's professional district teams.

Edinburgh Reivers: Lang, Milligan, Paterson, Utterson, Sharman, Hodge, Fairley, Stewart, Scott, Proudfoot, Lucking, Fullarton, Mather, Leslie, Hogg.

Edinburgh Reivers: C Paterson, K Milligan, J Hita, G Shiel, D Lee, D Hodge, G Burns, A Jacobsen, S Scott, B Stewart, A Lucking, I Fullarton, C Mather, C Hogg (G Hayter, 54 min), G Dall.

Glasgow Caledonians: G Metcalfe, S Longstaff, A Bulloch, J Stuart, J Craig, T Hayes, A Nicol, D Hilton, G Scott, G McIlwham, S Campbell (M Waite, 63), J White, R Reid (D Hall, 65), G Simpson, D Macfadyen (J Petrie 28).

Edinburgh Reivers: G Kiddie; K Milligan, J Hita, G Shiel, K Utterson; G Ross, I Fairley; A Jacobsen, S Scott, B Stewart, A Lucking, I Fullarton, C Mather, G Hayter, G Dall.