The 1999–2000 Ukrainian Cup was the ninth annual edition of Ukraine's football knockout competition, known as the Ukrainian Cup.
The winner of this competition was Dynamo Kyiv, beating FC Kryvbas Kryvyi Rih in the final.
Those finalists later also played a final game for the competition's trophy in May 2000.
All draws held at FFU headquarters (Building of Football) in Kyiv unless stated otherwise.
This article about a Ukrainian association football competition is a stub.