The 1999 Canisius Golden Griffins football team represented Canisius College as a member of the Metro Atlantic Athletic Conference (MAAC) during the 1999 NCAA Division I-AA football season.
Led by Chuck Williams in his fifth and final season as head coach, the Golden Griffins compiled an overall record of 1–10 with a mark of 0–8 in conference play, placing last out of nine teams in the MAAC.
The Canisius offense scored 133 points while the defense allowed 431 points.